Journal: International Journal of Molecular Sciences
Article Title: The Role of the Bone Morphogenetic Protein Antagonist Noggin in Nucleus Pulposus Intervertebral Disc Cells
doi: 10.3390/ijms252111803
Figure Lengend Snippet: Low expression of Noggin in human NPCs induced apoptosis and inhibited cell growth: ( A ) The proliferation of human NPCs was limited when transduced by Lenti-shNoggin. ( B ) Quantification of cell apoptosis by Hoechst 33258 dye on microscopy images. ( C ) Quantitative analysis for apoptosis by nucleus counting. (Apoptosis under Hoechst 33258 dye: bright blue, as the white arrows indicate. Scale bar: 250 μm. Shown are Means ± SEM. p -value, ns >0.05; * <0.05; n = 3–5).
Article Snippet: They were assessed using a reagent kit for the quantification of cell proliferation (TACS ® XTT, R&D Systems, Inc., Minneapolis, MN, USA) according to the manufacturer’s instructions.
Techniques: Expressing, Microscopy